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17-344

Sigma-Aldrich

H2A.X磷酸化检测试剂盒(流式细胞术)

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X.

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UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.32

质量水平

制造商/商品名称

Upstate®

技术

activity assay: suitable
flow cytometry: suitable

NCBI登记号

UniProt登记号

检测方法

fluorometric

运输

dry ice

相关类别

一般描述

组蛋白变体H2A.X的磷酸化是对双链DNA断裂的快速而敏感的反应。H2A.X磷酸化检测试剂盒(流式细胞仪)是一种基于细胞的检测试剂盒,用于流式细胞术检测磷酸化组蛋白H2A.X的水平。

H2A.X磷酸化检测试剂盒(流式细胞仪)是一种基于细胞的检测试剂盒,用于流式细胞术检测磷酸化组蛋白H2A.X的水平。在微孔板中培养细胞,并用诱导DNA损伤或凋亡(刺激H2A.X磷酸化)的试剂处理。然后将细胞固定并透化,以准备染色和检测。通过添加抗磷酸组蛋白H2A.X,FITC结合物来检测在丝氨酸139处磷酸化的组蛋白H2A.X。 然后在流式细胞仪中扫描细胞以定量磷酸化组蛋白H2A.X染色阳性的细胞数量。

应用

研究子类别
神经退行性疾病
研究类别
神经科学

包装

100次测定

组分

抗磷酸H2A.X,FITC结合物

正常小鼠IgG,FITC结合物(目录号12-487)

16X固定液

10X透化液

10X洗涤液

质量

组蛋白H2A.X的磷酸化

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

警示用语:

Danger

危险分类

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

靶器官

Eyes,Central nervous system, Respiratory system

WGK

WGK 3


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