推荐产品
质量水平
制造商/商品名称
Upstate®
技术
activity assay: suitable
flow cytometry: suitable
NCBI登记号
UniProt登记号
检测方法
fluorometric
运输
dry ice
相关类别
一般描述
组蛋白变体H2A.X的磷酸化是对双链DNA断裂的快速而敏感的反应。H2A.X磷酸化检测试剂盒(流式细胞仪)是一种基于细胞的检测试剂盒,用于流式细胞术检测磷酸化组蛋白H2A.X的水平。
H2A.X磷酸化检测试剂盒(流式细胞仪)是一种基于细胞的检测试剂盒,用于流式细胞术检测磷酸化组蛋白H2A.X的水平。在微孔板中培养细胞,并用诱导DNA损伤或凋亡(刺激H2A.X磷酸化)的试剂处理。然后将细胞固定并透化,以准备染色和检测。通过添加抗磷酸组蛋白H2A.X,FITC结合物来检测在丝氨酸139处磷酸化的组蛋白H2A.X。 然后在流式细胞仪中扫描细胞以定量磷酸化组蛋白H2A.X染色阳性的细胞数量。
H2A.X磷酸化检测试剂盒(流式细胞仪)是一种基于细胞的检测试剂盒,用于流式细胞术检测磷酸化组蛋白H2A.X的水平。在微孔板中培养细胞,并用诱导DNA损伤或凋亡(刺激H2A.X磷酸化)的试剂处理。然后将细胞固定并透化,以准备染色和检测。通过添加抗磷酸组蛋白H2A.X,FITC结合物来检测在丝氨酸139处磷酸化的组蛋白H2A.X。 然后在流式细胞仪中扫描细胞以定量磷酸化组蛋白H2A.X染色阳性的细胞数量。
应用
研究子类别
神经退行性疾病
神经退行性疾病
研究类别
神经科学
神经科学
包装
100次测定
组分
抗磷酸H2A.X,FITC结合物
正常小鼠IgG,FITC结合物(目录号12-487)
16X固定液
10X透化液
10X洗涤液
正常小鼠IgG,FITC结合物(目录号12-487)
16X固定液
10X透化液
10X洗涤液
质量
组蛋白H2A.X的磷酸化
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
警示用语:
Danger
危险分类
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
靶器官
Eyes,Central nervous system, Respiratory system
WGK
WGK 3
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Hypoxia (lack of oxygen) is a physiological stress often associated with solid tumors. Hypoxia correlates with poor prognosis since hypoxic regions within tumors are considered apoptosisresistant. Autophagy (cellular "self digestion") has been associated with hypoxia during cardiac ischemia and metabolic
Mutation research, 734(1-2), 20-29 (2012-05-09)
Berberine has been shown to possess anti-tumor activity against a wide spectrum of cancer cells. It inhibits cancer cell proliferation by inducing cell cycle arrest, at G1 and/or G2/M, and apoptosis. While it has been documented that berberine induces G1
RSC advances, 10(49), 29311-29319 (2020-08-07)
Immunofluorescence (IF) is a common method used in cell biology. The conventional protocol for IF staining is time and labor-intensive, operator dependent and reagent-consuming. Magnetic Bead (MB)-based microdevices are frequently utilized in cellular assays, but integration of simple and efficient
PLoS biology, 19(11), e3001471-e3001471 (2021-11-18)
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