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质量水平
制造商/商品名称
Upstate®
技术
activity assay: suitable (kinase)
NCBI登记号
UniProt登记号
运输
wet ice
应用
MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.
Used to detect/quantify: MAP Kinase/Erk
组分
Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
Inhibitor Cocktail (Cat.# 20-116)
Magnesium/ATP Cocktail (Cat.# 20-113)
Anti-phospho-MBP, clone P12 (Cat.# 05-429)
MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
Inhibitor Cocktail (Cat.# 20-116)
Magnesium/ATP Cocktail (Cat.# 20-113)
Anti-phospho-MBP, clone P12 (Cat.# 05-429)
质量
The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody.
其他说明
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
European journal of immunology, 24(11), 2653-2658 (1994-11-01)
The major purpose of this study was to elucidate Th1 [interferon (IFN)-gamma and interleukin (IL)-2] and Th2 (IL-4, IL-5 and IL-6) cytokine-producing CD3+ T cells in salivary glands, which are the major mucosal effector tissues in the oral region. Thus
Biochemical and biophysical research communications, 134(2), 671-677 (1986-01-29)
Human growth hormone was labelled with 125 Iodine by the stoichiometric modification of the chloramine-T method to a specific activity of 50-80 microCi/microgram, and the iodinated mixture was purified by reverse-phase high performance liquid chromatography using a C18 column (SynChropak
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Nanomechanical resonators have shown potential application for mass sensing and have been used to detect a variety of biomolecules. In this study, a dynamic resonance-based technique was used to detect prion proteins (PrP), which in conformationally altered forms are known
PloS one, 3(12), e3873-e3873 (2008-12-05)
The Extracellular Regulated Kinase 1 and 2 transduce a variety of extracellular stimuli regulating processes as diverse as proliferation, differentiation and synaptic plasticity. Once activated in the cytoplasm, ERK1 and ERK2 translocate into the nucleus and interact with nuclear substrates
Stem cell reports, 11(4), 929-943 (2018-09-25)
Tyrosine kinase inhibitors (TKi) are effective against chronic myeloid leukemia (CML), but their inefficacy on leukemia stem cells (LSCs) may lead to relapse. To identify new druggable targets alternative to BCR/ABL, we investigated the role of the MEK5/ERK5 pathway in
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