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Merck
CN
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主要文件

安全信息

171536

Sigma-Aldrich

AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda

full length enzyme consisting of subunits A1, B1, and G1

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.77

重组

expressed in insect cells (S. frugiperda)

质量水平

100

方案

≥95% (SDS-PAGE)

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−70°C

一般描述

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).
M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

警告

Toxicity: Standard Handling (A)

外形

In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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