推荐产品
生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
4G10®, monoclonal
种属反应性(根据同源性预测)
all
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
同位素/亚型
IgG2bκ
运输
wet ice
靶向翻译后修饰
phosphorylation (pTyr)
基因信息
human ... PID1(55022)
一般描述
1989年抗磷酸酪氨酸克隆4G10的开发对研究人员来说是一个巨大的发现。4G10是检测酪氨酸磷酸化的第一个也是最佳单克隆抗体。4G10以其灵敏度和检测多种底物上多种酪氨酸磷酸化的能力而闻名。在过去的20年中,它已经在几乎每个应用和酪氨酸靶标中得到了数千名科学和医学研究人员的验证。现在Millipore用4G10铂做到最好甚至更好。 我们将4G10 与下一个最受推崇的抗磷酸酪氨酸克隆PY20合并在一起,制成4G10 铂。PY20本身是4G10的非常差的替代品,但是它的累加效应允许在更多的底物上进行更高水平的检测,而即使单独使用4G10也能做到这一点。
免疫原
4G10的免疫原使用与KLH偶联的磷酸酪胺,PY20的免疫原使用与载体蛋白偶联的磷酸酪氨酸。
应用
4G10铂,抗磷酸酪氨酸,琼脂糖结合物可检测磷酸酪氨酸蛋白的水平&已发表&并经验证用于IP。
特点和优势
形式:凝胶固定
质量
免疫沉淀:
使用20 μl该试剂的50%浆液(10 μl包装的珠子)从250 μg PDGF刺激的NIH3T3细胞裂解物或EGF刺激的A431细胞裂解物中免疫沉淀酪氨酸磷酸化的蛋白。
使用20 μl该试剂的50%浆液(10 μl包装的珠子)从250 μg PDGF刺激的NIH3T3细胞裂解物或EGF刺激的A431细胞裂解物中免疫沉淀酪氨酸磷酸化的蛋白。
目标描述
依赖于酪氨酸磷酸化蛋白的分子量
外形
以琼脂糖偶联的,蛋白G纯化的小鼠单克隆4G10(IgG2bκ)和蛋白A纯化的PY20(IgG2b)的专有混合物的50%浆液形式提供,溶于PBS(pH 7.4)和0.05%叠氮化钠。
蛋白G纯化的小鼠单克隆4G10(IgG2bκ)和蛋白A纯化的PY20(IgG2b)
储存及稳定性
自发货之日起在2-8°C下可稳定保存6个月。 为了最大程度地回收产品,在取下盖子之前,将样品瓶进行离心。
注:不可冷冻。
注:不可冷冻。
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
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