生物来源
mouse
质量水平
偶联物
peroxidase conjugate
抗体产品类型
primary antibodies
克隆
4G10®, monoclonal
PY20, monoclonal
种属反应性（根据同源性预测）
all
制造商/商品名称
Upstate®
技术
ELISA: suitable
western blot: suitable
同位素/亚型
IgG2b
运输
wet ice
靶向翻译后修饰
phosphorylation (pTyr)
基因信息
human ... PID1(55022)
一般描述
1989年抗磷酸酪氨酸克隆4G10的开发对研究人员来说是一个巨大的发现。4G10是第一个检测酪氨酸磷酸化的单克隆抗体，是检测酪氨酸磷酸化的最佳单克隆抗体。4G10以其灵敏度和检测多种底物上多种酪氨酸磷酸化的能力而闻名。在过去的20年中，它已经在几乎每个应用和酪氨酸靶标中得到了数千名科学和医学研究人员的验证。为了改进一百种尝试过但没有人成功的东西，我们将4G10 与下一个最受推崇的抗磷酸酪氨酸克隆PY20合并在一起，制成4G10 铂。PY20本身是4G10的非常差的替代品，但是它的累加效应可在更多的底物上进行更高水平的检测，甚至可以单独使用4G10 。
应用
使用抗磷酸酪氨酸抗体，4G10铂，HRP偶联物（小鼠单克隆抗体）检测磷酸酪氨酸，该抗体已在WB，ELISA中显示有效。
质量
通过蛋白质印迹法进行了常规评估。
目标描述
变化
外形
100 μL经蛋白G纯化的小鼠单克隆4G10（IgG2bƙ）HRP偶联物和经蛋白A纯化的小鼠单克隆PY20，HRP偶联物（IgG2b）的专有混合物，溶于含有0.05% Kathon和HRP稳定化合物的PBS中。如果在10 mL封闭缓冲液中以1:2000 – 1:4000的最佳浓度使用，则足以进行最少10次免疫印迹。 液体。
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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危险分类
Aquatic Chronic 2 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
does not flash
闪点(°C)
does not flash
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