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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
4G10®, monoclonal
种属反应性（根据同源性预测）
all
技术
immunoprecipitation (IP): suitable
同位素/亚型
IgG2bκ
运输
wet ice
靶向翻译后修饰
phosphorylation (pTyr)
基因信息
human ... PID1(55022)
一般描述
Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
特异性
This antibody recognizes tyrosine-phosphorylated proteins from all species
免疫原
Phosphotyramine-KLH
应用
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
质量
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).
(Lane 2).
目标描述
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
外形
Protein G-Sepharose Chromatography
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Format: Purified
储存及稳定性
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
Note: DO NOT FREEZE
分析说明
Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
M Whitfield et al.
Reproduction (Cambridge, England), 154(6), 827-842 (2017-10-04)
Lipid metabolism disorders (dyslipidemia) are causes of male infertility, but little is known about their impact on male gametes when considering post-testicular maturation events, given that studies concentrate most often on endocrine dysfunctions and testicular consequences. In this study, three-month-old
F Saez et al.
Andrology, 7(5), 654-661 (2019-06-05)
Lipid metabolic disorders (dyslipidemia) are constantly increasing in occidental societies and lead to the development of pathologies such as obesity, diabetes, and metabolic syndrome. It has been demonstrated that dyslipidemia can alter the reproductive function. Animal models have recently been
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