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16-256

Sigma-Aldrich

抗磷脂酰丝氨酸抗体,克隆1H6,Alexa Fluor 488

clone 1H6, Upstate®, from mouse

别名:

488-Alexa Fluor Antibody, Alexa Fluor 488 Detection, Clone 1H6 Anti-Phosphatidylserine

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

ALEXA FLUOR 488

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

1H6, monoclonal

种属反应性

vertebrates

制造商/商品名称

Upstate®

技术

flow cytometry: suitable

同位素/亚型

IgG

运输

wet ice

靶向翻译后修饰

phosphorylation (pSer)

一般描述

意义:
抗磷脂酰丝氨酸可用于检测膜磷脂磷脂酰丝氨酸(PS)从内膜小叶向外膜小叶的移位;它提供了膜联蛋白V的替代方法,用于定量细胞凋亡。
磷脂酰丝氨酸或PS是天然存在的磷脂营养素。PS对所有人体细胞的功能至关重要,但最集中在大脑中。它在该器官中的相对丰度反映了它已被证明参与了各种神经细胞功能,包括神经递质释放和突触活性。临床研究表明,PS可以支持随着年龄增长而下降的大脑功能。

特异性

识别细胞膜中的磷脂酰丝氨酸(PS)

免疫原

含有70%磷脂酰丝氨酸和30%磷脂酰甘油的脂质体。

应用

使用经验证可用于FC的抗磷脂酰丝氨酸抗体(克隆1H6,Alexa Fluor 488)检测磷脂酰丝氨酸。
研究子类别
凋亡-附加
研究类别
细胞凋亡 & 癌症

质量

流式细胞术: 0.2 μg该抗体可在固定Jurkat细胞中检测出磷脂酰丝氨酸(见第2页)。

凋亡测定:使用抗磷脂酰丝氨酸,克隆1H6,Alexa Fluor 488偶联物或膜联蛋白V FITC偶联物测量星形孢菌素对Jurkat细胞中凋亡诱导的时间过程。

外形

纯化的小鼠单克隆IgG,溶于含有PBS、1% BSA、0.05%吐温和0.05%叠氮化钠的缓冲液中。 在4ºC为液体形式。
蛋白G纯化

储存及稳定性

自收到之日起可稳定保存1年。

分析说明

对照
阴性对照:目录号16-240,正常小鼠IgG,Alexa Fluor 488-偶联物。

其他说明

浓度:请参考批次特异性浓缩物的检验报告。

法律信息

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Alexa Fluor是Molecular Probes, Inc.的注册商标。

除非在产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得对人或动物用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用。

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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访问文档库

Yi An et al.
Scientific reports, 11(1), 6392-6392 (2021-03-20)
Head and neck squamous cell carcinomas (HNSCC) induced by human papillomavirus (HPV) have increased recently in the US. However, the distinct alterations of molecules involved in the death pathways and drug effects targeting inhibitor of apoptosis proteins (IAPs) have not
Carlos E Bravo-Iñiguez et al.
Nature communications, 14(1), 3122-3122 (2023-06-02)
Deficiency of coagulation factor VIII in hemophilia A disrupts clotting and prolongs bleeding. While the current mainstay of therapy is infusion of factor VIII concentrates, inhibitor antibodies often render these ineffective. Because preclinical evidence shows electrical vagus nerve stimulation accelerates
Yuta Nakazawa et al.
eLife, 10 (2021-11-10)
Although tumor-infiltrating regulatory T (Treg) cells play a pivotal role in tumor immunity, how Treg cell activation are regulated in tumor microenvironments remains unclear. Here, we found that mice deficient in the inhibitory immunoreceptor CD300a on their dendritic cells (DCs)
I Vermes et al.
Journal of immunological methods, 184(1), 39-51 (1995-07-17)
In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane
Sadna Budhu et al.
Cell reports, 34(2), 108620-108620 (2021-01-14)
Phosphatidylserine (PS) is exposed on the surface of apoptotic cells and is known to promote immunosuppressive signals in the tumor microenvironment (TME). Antibodies that block PS interaction with its receptors have been shown to repolarize the TME into a proinflammatory

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实验方案

了解流式细胞分析实验方案的主要步骤,以便轻松完成后续流式细胞分析实验。

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