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Merck
CN
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主要文件

16-201

Sigma-Aldrich

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose G beads

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About This Item

UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.32

质量水平

表单

liquid

制造商/商品名称

Upstate®

技术

ChIP: suitable

适用性

suitable for immunoprecipitation

运输

wet ice

一般描述

重组蛋白G对IgG分子的Fc区具有非常高的亲和力。将蛋白G固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。
重组蛋白G通过溴化氰键与琼脂糖共价结合。当收集用于染色质免疫沉淀(ChIP分析)的免疫复合物时,超声处理的鲑鱼精子DNA会阻断蛋白G琼脂糖上的非特异性DNA结合位点。

包装

2.5 mL填充珠以50%凝胶浆形式提供,最终体积为5 mL。

质量

在染色质免疫沉淀中常规评估,用于预先清除丁酸钠处理的HeLa细胞的上清液,以及随后与乙酰化组蛋白交联的DNA的免疫沉淀

外形

含有1 mg超声鲑鱼精子DNA、2.5 mg BSA和约5 mg重组蛋白G的2.5 mL填充珠,以50%凝胶浆形式提供,每瓶最终体积为5 mL。 混悬在含有0.05%叠氮化钠的10 mM Tris-HCl,1 mM EDTA(pH 8.0)中。 液体混悬液。

储存及稳定性

自发货之日起在4°C下可保存1年。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 1


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Kyster K Nanan et al.
Nucleic acids research, 45(22), 12780-12797 (2017-12-16)
Actively transcribed genes adopt a unique chromatin environment with characteristic patterns of enrichment. Within gene bodies, H3K36me3 and cytosine DNA methylation are elevated at exons of spliced genes and have been implicated in the regulation of pre-mRNA splicing. H3K36me3 is
Kyung-Soon Park et al.
Stem cells (Dayton, Ohio), 31(2), 282-292 (2012-11-22)
Self-renewal and pluripotency are hallmark properties of pluripotent stem cells, including embryonic stem cells (ESCs) and iPS cells. Previous studies revealed the ESC-specific core transcription circuitry and showed that these core factors (e.g., Oct3/4, Sox2, and Nanog) regulate not only
Hidenori Kaneoka et al.
Cytotechnology, 57(2), 123-128 (2008-11-13)
The glucocorticoid receptor regulates liver-specific expression of the tryptophan oxygenase gene through glucocorticoid responsive elements located -0.45 and -1.2 kb from the transcription start site. However, the hormone-mediated induction is restricted to adult hepatocytes, and fetal hepatocytes are unable to
Nikita Savelyev et al.
Oncotarget, 9(27), 18712-18719 (2018-05-04)
Gallotannin (or tannic acid) is a naturally occurring compound that inhibits cell growth and activity of different DNA-polymerases, including telomerase. The purpose of the present study was to gain insight into the mechanism of telomerase inhibition by gallotannin. We determined
Yang Tang et al.
Molecular plant, 10(2), 260-273 (2016-11-24)
Light is a crucial environmental signal that promotes photomorphogenesis, the developmental process with a series of light-dependent alterations for plants to adapt various external challenges. Chromatin modification has been proposed to be involved in such light-mediated growth, but the underlying

相关内容

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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