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Merck
CN
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主要文件

安全信息

16-157

Sigma-Aldrich

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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About This Item

UNSPSC代码:
12352202
eCl@ss:
32160405
NACRES:
NA.32

生物来源

Staphylococcus aureus

质量水平

表单

liquid

制造商/商品名称

Upstate®

技术

ChIP: suitable

适用性

suitable for immunoprecipitation

运输

wet ice

一般描述

与含有经超声处理的鲑鱼精子DNA的琼脂糖通过烷基胺键共价结合的蛋白A。 鲑鱼精子DNA可封闭与染色质IP(ChIP)分析用微珠的非特异性相互作用。请注意ChIP试剂盒中的组分号16-157C不可单独订购,请购买16-157。
来自金黄色葡萄球菌的蛋白A对于IgG分子的Fc区具有非常高的亲和力。将蛋白A固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。

应用

适合用于染色质免疫沉淀(ChIP)分析

质量

在染色质免疫沉淀中通过拉下与乙酰化组蛋白交联的DNA而进行常规评估; 后续的检测是采用ChIPs级(06-866)抗-乙酰化组蛋白H4抗体进行的

外形

含有0.05%叠氮化钠的10mM Tris-HCl, 1mM EDTA, pH 8.0溶液。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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Generation of p53 target database via integration of microarray and global p53 DNA-binding site analysis.
Suxing Liu, Asra Mirza, Luquan Wang
Methods in Molecular Biology null
Mohammad Kamran et al.
Journal of immunology (Baltimore, Md. : 1950), 205(12), 3311-3318 (2020-11-15)
IL-13 plays a critical role in mediating many biological processes responsible for allergic inflammation. Mast cells express Il13 mRNA and produce IL-13 protein in response to antigenic stimulation. Enhancers are essential in promoting gene transcription and are thought to activate
Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
Lauren J Buro,Shaili Shah,Melissa A Henriksen
Journal of Visualized Experiments null
Yurong Xie et al.
Nature communications, 11(1), 1955-1955 (2020-04-25)
Branching/tillering is an important parameter of plant architecture and is tightly regulated by both internal factors (such as plant hormones) and external factors (such as light conditions). How the various signaling pathways converge to coordinately regulate branching is not well
In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.
Foster JG, Cassida KA, Turner KE
Veterinary Parasitology null

相关内容

蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

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