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表单
liquid
制造商/商品名称
Upstate®
技术
affinity chromatography: suitable
immunoprecipitation (IP): suitable
western blot: suitable
运输
wet ice
一般描述
蛋白质A是一种免疫球蛋白(Ig)结合蛋白，用于纯化大量IgG。它与抗体的Fc部分在CH2–CH3界面结合。蛋白质A-琼脂糖可用于低压抗体分离。
重组蛋白A，共价结合到高度交叉链接的6%琼脂糖磁珠。
结合量 40mg 人 IgG/ml 琼脂糖
重组蛋白A，共价结合到高度交叉链接的6%琼脂糖磁珠。
结合量 40mg 人 IgG/ml 琼脂糖
应用
蛋白A琼脂糖，Fast Flow已被用于免疫沉淀和染色质免疫沉淀(ChIP)。
质量
在免疫沉淀中进行了常规评估
外形
含有 0.01％ 硫柳汞的无菌蒸馏水
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
Vasavi Sundaram et al.
Genome research, 24(12), 1963-1976 (2014-10-17)
Transposable elements (TEs) have been shown to contain functional binding sites for certain transcription factors (TFs). However, the extent to which TEs contribute to the evolution of TF binding sites is not well known. We comprehensively mapped binding sites for
Yong Cheng et al.
Nature, 515(7527), 371-375 (2014-11-21)
To broaden our understanding of the evolution of gene regulation mechanisms, we generated occupancy profiles for 34 orthologous transcription factors (TFs) in human-mouse erythroid progenitor, lymphoblast and embryonic stem-cell lines. By combining the genome-wide transcription factor occupancy repertoires, associated epigenetic
Suzana Markolovic et al.
Nature chemical biology, 14(7), 688-695 (2018-06-20)
Biochemical, structural and cellular studies reveal Jumonji-C (JmjC) domain-containing 7 (JMJD7) to be a 2-oxoglutarate (2OG)-dependent oxygenase that catalyzes (3S)-lysyl hydroxylation. Crystallographic analyses reveal JMJD7 to be more closely related to the JmjC hydroxylases than to the JmjC demethylases. Biophysical
Chih-Chin Huang et al.
The Journal of biological chemistry, 284(25), 17206-17215 (2009-04-15)
G protein-coupled receptor (GPCR) kinases (GRKs) phosphorylate activated GPCRs and initiate their desensitization. Many prior studies suggest that activated GPCRs dock to an allosteric site on the GRKs and thereby stimulate kinase activity. The extreme N-terminal region of GRKs is
Zhiqiang Wu et al.
Nucleic acids research, 43(6), 3167-3179 (2015-03-05)
The activation of NF-κB has emerged as an important mechanism for the modulation of the response to DNA double-strand breaks (DSBs). The concomitant SUMOylation and phosphorylation of IKKγ by PIASy and ATM, respectively, is a key event in this mechanism.
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