产品名称
Chromolith® Si硅胶色谱柱, L × I.D. 100 mm x 10 mm column
material
PEEK column
agency
suitable for USP L3
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
parameter
150 bar pressure
45 °C max. temp.
technique(s)
HPLC: suitable (Semi Prep HPLC)
LC/MS: suitable
column L × I.D.
10 cm × 10 mm
matrix
monolithic silica
matrix active group
Silica bonding phase
pore size
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
storage temp.
no temp limit
Quality Level
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Analysis Note
Pressure: ≤ 25 bar
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95
General description
Chromolith® SemiPrep Si HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles.
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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