产品名称
Purospher® STAR Phenyl (5 µm), L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column
material
stainless steel column
agency
suitable for USP L11
product line
Purospher®
feature
endcapped: no
packaging
1 ea of
extent of labeling
12.5% Carbon loading
parameter
400 bar pressure
65 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
25 cm × 4.6 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
Phenyl bonding phase
particle size
5 μm
pore size
120 Å pore size
operating pH range
1.5-10.5
separation technique
reversed phase
supercritical fluid (SFC)
storage temp.
no temp limit
Quality Level
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Application
Intended to be used in the separation of antibiotics, aromatic amines, flavonoids, ketones, nucleosides, PAHs, peptides, preservatives, steroids, metabolized steroids, and sweeteners.
Features and Benefits
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Enhanced selectivity for aromatic compounds
- Suitable for use with highly aqueous mobile phases
- Good suitability for LC-MS applications
General description
Purospher® STAR Phenyl, 5 µm UHPLC columns are the best alternative to RP-8 or RP-18 columns for the efficient separation of aromatic compounds, and compounds containing aromatic groups. Purospher® STAR Phenyl can retain analytes via several different mechanisms, including ο-ο interactions between the overlap of the delocalized electrons on the analyte and the stationary phase phenyl group, and via partitioning between the mobile phase and the hydrophobic aryl-alkyl phase. Our Purospher® STAR Phenyl columns are based on high purity silica particles, which provide symmetrical peaks for basic compounds, as well as high stability and excellent reproducibility. Furthermore, hydrophobic compounds elute much faster with Purospher® STAR Phenyl columns than with C-18 columns.Purospher® STAR Phenyl, 5 µm Hibar® RT 250-4.6 is a ready to use HPLC column suitable for connection to any HPLC system. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for HPLC high-resolution separation of complex samples.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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