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1.51466

Supelco

Chromolith® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Columns Validation Kit (3 Columns from 3 different batches)

别名:

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC columns Validation Kit (3 columns from 3 different batches)

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About This Item

UNSPSC代码:
41115709
NACRES:
SB.52

产品名称

Chromolith® RP-18封端, L × I.D. 100 mm x 4.6 mm HPLC columns Validation Kit (3 columns from 3 different batches)

物料

PEEK column

质量水平

100

Agency

suitable for USP L1

产品线

Chromolith®

特点

endcapped

包装

pkg of 3 ea

标记范围

18% Carbon loading

参数

200 bar pressure
45 °C max. temp.

技术

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

色谱柱长度 × 内径

10 cm × 4.6 mm

基质

monolithic silica

基质活性基团

C18 bonding phase

孔径

13 nm mesoporosity
2 μm macroporosity

工作pH范围

2-7.5

分离技术

reversed phase

储存温度

no temp limit

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一般描述

Chromolith® Performance RP-18 endcapped 100-4.6 HPLC columns Validation Kit consists of 3 columns from 3 different manufacturing batches. Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® Performance RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), and the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:
  • Rapid separations at very low column back-pressure
  • High matrix tolerance, allowing time and cost savings with simplified workflows
  • Cost-savings due to extended column lifetimes
  • Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are: Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]. The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467] Need more efficiency? Chromolith® High Resolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter. Learn more:Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC ColumnsAll Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.Supelco HPLC Products: The Best Choice for Any LC Instrument

应用


  • Rapid Determination of 30 Polyphenols in Tongmai Formula: This study utilized the Chromolith® RP-18 endcapped column in a liquid chromatography-tandem mass spectrometry setup to analyze 30 polyphenolic compounds in a traditional Chinese medicine formula, demonstrating its application in pharmaceutical analysis and quality control of complex herbal formulations (Wang et al., 2017).

  • Ultra-fast gradient LC method for omeprazole analysis using a monolithic column: The Chromolith® RP-18 endcapped column was employed in an ultra-fast gradient liquid chromatography method for the analysis of omeprazole in enteric-coated pellets, highlighting its efficiency in pharmaceutical quality control and rapid method development (Borges et al., 2010).

分析说明

Extent of testing: passes test

法律信息

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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商品

Monolithic Silica with Bimodal Pore Structure

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

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