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1.50487

Supelco

SeQuant® ZIC®-HILIC 100Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 1 mm capillay Column

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别名:
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
UNSPSC代码:
41115709

product name

SeQuant® ZIC-HILIC™ (3.5µm,100Å) 150 x 1 mm, suitable for HPLC

物料

stainless steel column

质量水平

100

Agency

suitable for USP L114

产品线

SeQuant®

特点

endcapped: no

参数

400 bar pressure
70 °C max. temp.

技术

HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)

色谱柱长度 × 内径

15 cm × 1 mm

基质

fully porous particle

基质活性基团

ZIC-HILIC bonding phase

粒径

3.5 μm

孔径

100 Å pore size

工作pH值

2-7.5

分离技术

hydrophilic interaction (HILIC)

储存温度

no temp limit

相关类别

一般描述

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 150 mm length x 1 mm ID is the optimal choice for the sensetive separation of complex samples under capillary LC-MS conditions.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

法律信息

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation
Arena K, et al.
Molecules (Basel), 27(5), 1727-1727 (2022)
Katia Arena et al.
Analytical and bioanalytical chemistry, 415(13), 2371-2382 (2022-07-15)
In this work, a comprehensive two-dimensional liquid chromatography system, comprised of a ZIC-HILIC and C18 columns in the first and second dimensions, respectively, was tuned and employed for attaining high resolution profiles of the polyphenolic pattern in seven commercial berry

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