推荐产品
产品名称
SeQuant® ZIC-HILIC™ (5µm,200Å) PEEK 50 x 4.6 mm, suitable for HPLC
物料
PEEK-lined stainless steel column
质量水平
Agency
suitable for USP L114
产品线
SeQuant®
特点
endcapped: no
参数
500 bar pressure
70 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
色谱柱长度 × 内径
5 cm × 4.6 mm
基质
fully porous particle
基质活性基团
ZIC-HILIC bonding phase
粒径
5 μm
孔径
200 Å pore size
工作pH值
2-7.5
分离技术
hydrophilic interaction (HILIC)
储存温度
no temp limit
一般描述
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar columns using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar columns using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
分析说明
Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40
Symmetry (Cytosine): 0.95 - 1.40
法律信息
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
实验方案
Separation of EDTA
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