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物料
PEEK-lined stainless steel column
Agency
suitable for USP L114
产品线
SeQuant™
特点
endcapped: no
参数
550 bar pressure
70 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
色谱柱长度 × 内径
2 cm × 2.1 mm
基质
fully porous particle
基质活性基团
ZIC-HILIC bonding phase
粒径
3.5 μm
孔径
100 Å pore size
工作pH值
2-7.5
分离技术
hydrophilic interaction (HILIC)
储存温度
2-30°C
一般描述
SeQuant™ ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.
The column dimension of 20 mm length x 2.1 mm ID is the optimal choice for the rapid separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances. SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.
The column dimension of 20 mm length x 2.1 mm ID is the optimal choice for the rapid separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances. SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
分析说明
Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.30
Symmetry (Cytosine): 0.95 - 1.30
法律信息
SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
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