product name
Purospher® STAR RP-18 封端 (3 µm), L × I.D. 125 mm x 3 mm Hibar® RT HPLC column
物料
stainless steel column
质量水平
Agency
suitable for USP L1
产品线
Purospher®
特点
endcapped
包装
1 ea of
标记范围
17% Carbon loading
参数
400 bar pressure
65 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
色谱柱长度 × 内径
12.5 cm × 3 mm
表面积
330 m2/g
基质
fully porous particle
基质活性基团
C18 bonding phase
粒径
3 μm
孔径
120 Å pore size
operating pH range
1.5-10.5
分离技术
reversed phase
supercritical fluid (SFC)
储存温度
no temp limit
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相关类别
一般描述
Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 3 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 125-3 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 125 mm length x 3 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivity. A 3 mm I.D. column enables a more sustainable separations in comparison to 4 or 4.6 mm I.D. column due to the use of less solvent for the chromatographic separation.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001]. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
应用
Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.
特点和优势
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Reproducible results from run-to-run and batch-to-batch
- Best all-round performance according to Tanaka
- Outstanding pH stability from pH 1.5 to 10.5
- No phase collapse when using highly aqueous mobile phases
- Good suitability for LC-MS applications
分析说明
Pressure: ≤ 120 bar
Theoretical Plates (N/m) (Anthracene): ≥ 130000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 6.0
Theoretical Plates (N/m) (Anthracene): ≥ 130000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 6.0
法律信息
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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