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Merck
CN

1.50245

Purospher® STAR NH2 (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

别名:

Purospher® STAR NH2 (5 µm) LiChroCART™ 250-4

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UNSPSC Code:
41115709
NACRES:
SB.52

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产品名称

Purospher® STAR NH2 (5 µm), L × I.D. 250 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use

material

stainless steel column

agency

suitable for USP L8

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

250 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

25 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

NH2 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

Quality Level

100

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Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): 0.5 – 1.8

Application

Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%)
  • Low silanol activity
  • Reproducible results from run-to-run and batch-to-batch

General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phaseconsisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART™ 250-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001. LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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