product name
Purospher® STAR NH2 (5 µm), L × I.D. 125 mm x 4 mm LiChroCART® HPLC cartridge, manuCART™ holder required for use
物料
stainless steel column
质量水平
Agency
suitable for USP L8
产品线
Purospher®
特点
endcapped: no
制造商/商品名称
Purospher®
包装
1 ea of
标记范围
3.5% Carbon loading
参数
250 bar pressure
技术
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
色谱柱长度 × 内径
12.5 cm × 4 mm
表面积
330 m2/g
基质
fully porous particle
基质活性基团
NH2 bonding phase
粒径
5 μm
孔径
120 Å pore size
operating pH range
2-7.5
分离技术
hydrophilic interaction (HILIC)
normal phase
reversed phase
储存温度
no temp limit
正在寻找类似产品? 访问 产品对比指南
一般描述
Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART® 125-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART® 4-4 part number 1.50267.0001 LiChroCART® cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 125 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
应用
Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.
特点和优势
- Ultra-pure silica (99.999%)
- Low silanol activity
- Reproducible results from run-to-run and batch-to-batch
分析说明
Pressure: ≤ 30 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
法律信息
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门