1.50244
Purospher® STAR NH2 (5 µm) HPLC Columns
L × I.D. 125 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
别名:
Purospher STAR NH2 (5 µm) LiChroCART™ 125-4
产品名称
Purospher® STAR NH2 (5 µm), L × I.D. 125 mm x 4 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
material
stainless steel column
agency
suitable for USP L8
product line
Purospher®
feature
endcapped: no
manufacturer/tradename
Purospher®
packaging
1 ea of
extent of labeling
3.5% Carbon loading
parameter
250 bar pressure
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
12.5 cm × 4 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
NH2 bonding phase
particle size
5 μm
pore size
120 Å pore size
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
reversed phase
storage temp.
no temp limit
Quality Level
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Application
Intended to be used in the separation of alcohols,carboxylic acids, fat soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water soluble vitamins.
Analysis Note
Pressure: ≤ 30 bar
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
Symmetry (2-Nitrotoluene): 0.8 - 1.4
Capacity factor (2-Nitrotoluene): ≤ 3.0
Features and Benefits
- Ultra-pure silica (99.999%)
- Low silanol activity
- Reproducible results from run-to-run and batch-to-batch
General description
Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm LiChroCART™ 125-4 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001 LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder is hand-tight only and have therefore a maximum pressure stability of 250 bar.The column dimension of 125 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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