14-856
Ubiquitin C-terminal hydrolase L3 (UCH-L3), 50 µg
From human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters & thioesters of the C-terminus of ubiquitin.
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所有图片(1)
UNSPSC代码:
12352200
eCl@ss:
32160405
NACRES:
NA.32
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生物来源
human
质量水平
重组
expressed in E. coli
比活
>1000 pmol/min-μg, 25 °C (with 1 μM ubiquitin-AMC as substrate and UCH-L3 at 20 pM (0.58 ng/mL).)
分子量
Mw 26.2 kDa
制造商/商品名称
Upstate®
NCBI登记号
UniProt登记号
运输
dry ice
一般描述
Produced from human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. UCH-L3 is a member of the lower molecular weight group of UCHs involved in the hydrolysis of small C-terminal derivatives of ubiquitin that form non-specifically during the process of protein ubiquitinylation.
Product Source: Recombinant human UCH-L3 expressed in E.coli.
应用
From human cDNA, expressed in E. coli. Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters & thioesters of the C-terminus of ubiquitin.
储存及稳定性
Store at -70°C for up to 12 months from date of receipt. As supplied, the enzyme is stable on ice for several hours. Activity is stable up to 6 freeze/thaw cycles (snap freezing in a dry/ice ethanol bath or liquid nitrogen).
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
K D Wilkinson et al.
Journal of molecular biology, 291(5), 1067-1077 (1999-10-16)
The ubiquitin fold is a versatile and widely used targeting signal that is added post-translationally to a variety of proteins. Covalent attachment of one or more ubiquitin domains results in localization of the target protein to the proteasome, the nucleus
Roles of ubiquitinylation in proteolysis and cellular regulation
Wilkinson, K D
Annual Review of Nutrition, 15, 161-189 (1995)
L J Kurihara et al.
Molecular and cellular biology, 20(7), 2498-2504 (2000-03-14)
Mice homozygous for the s(1Acrg) deletion at the Ednrb locus arrest at embryonic day 8.5. To determine the molecular basis of this defect, we initiated positional cloning of the s(1Acrg) minimal region. The mouse Uch-L3 (ubiquitin C-terminal hydrolase L3) gene
Shahram Misaghi et al.
The Journal of biological chemistry, 280(2), 1512-1520 (2004-11-09)
Ubiquitin C-terminal hydrolases (UCHs) comprise a family of small ubiquitin-specific proteases of uncertain function. Although no cellular substrates have been identified for UCHs, their highly tissue-specific expression patterns and the association of UCH-L1 mutations with human disease strongly suggest a
Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes.
Dang, L C, et al.
Biochemistry, 37, 1868-1879 (1998)
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