14-810
UbcH5b结合酶，100 µg
The His6-tagged fusion protein of UbcH5b is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
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About This Item
UNSPSC代码:
12352200
eCl@ss:
32160405
NACRES:
NA.32
生物来源
human
质量水平
检测方案
>95% (SDS-PAGE)
分子量
Mw 17 kDa
制造商/商品名称
Upstate®
NCBI登记号
UniProt登记号
运输
dry ice
基因信息
human ... UBE2D2(7322)
一般描述
UbcH5b的His6标记融合蛋白带电荷并支持体外泛素化。与GST标记的类似物不同，His6标记UbcH5家族成员在相似的条件下以相似的速率与泛素形成硫醇酯偶联物。该酶的His6标记形式不容易自泛素化。UbcH5b与酿酒酵母Ubc4/5 E2酶家族高度同源，被证明对降解调节蛋白和异常蛋白至关重要。UbcH5b与UbcH5a和UbcH5c一起是细胞提取物中最活跃的一类E2酶，并且与包括p53、NF-κB和c-Fos在内的许多转录因子的调节有关。
产品来源：在大肠埃希菌中表达的重组人UbcH5b结合酶。
储存及稳定性
自发货之日起在-80°C可稳定保存1年。避免反复冻融循环。初始解冻后，将等分试样装入单独的试管中，并在-80°C下重新冷冻。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.
Seufert, W and Jentsch, S
The Embo Journal, 9, 543-550 (1990)
J P Jensen et al.
The Journal of biological chemistry, 270(51), 30408-30414 (1995-12-22)
Two very closely related human E2 ubiquitin conjugating enzymes, UbfH5B and UbcH5C, have been identified. These enzymes are products of distinct genes and are 88-89% identical in amino acid sequence to the recently described human E2, UbcH5 (now designated UbcH5A)
H Gonen et al.
The Journal of biological chemistry, 274(21), 14823-14830 (1999-05-18)
The last step in the activation of the transcription factor NF-kappaB is signal-induced, ubiquitin- and proteasome-mediated degradation of the inhibitor IkappaBalpha. Although most of the components involved in the activation and degradation pathways have been identified, the ubiquitin carrier proteins
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