FGFR2 (N549H) Protein, active, 10 µg

N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.

Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Protein Target: FGFR2

Target Sub-Family: TK

Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).

Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.

Ni²⁺/NTA-agarose

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

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