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生物来源
human
质量水平
重组
expressed in E. coli
分子量
Mw 11 kDa
制造商/商品名称
Upstate®
技术
activity assay: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
dry ice
一般描述
在大肠杆菌中生产的重组人组蛋白H4。
质量
已作为体外酶反应的底物进行了常规评估。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Expression and purification of recombinant human histones.
Tanaka, Yoshinori, et al.
Methods, 33, 3-11 (2004)
Characterization of nucleosome core particles containing histone proteins made in bacteria.
Luger, K, et al.
Journal of Molecular Biology, 272, 301-311 (1997)
Matthew V Holt et al.
Current protocols, 1(2), e26-e26 (2021-02-04)
Histones are the primary protein component of chromatin and are involved in virtually all DNA-templated processes. Histones are abundantly post-translationally modified by a variety of chromatin-modifying machinery. These post-translational modifications (PTMs) are recognized by a range of "reader" proteins, which
Expression, purification, and structural characterization of human histone H4.
Vergani, Laura, et al.
Protein Expression and Purification, 24, 420-428 (2002)
David Shechter et al.
Nature protocols, 2(6), 1445-1457 (2007-06-05)
Histone proteins are the major protein components of chromatin, the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. Chromatin is the substrate of many biological processes, such as gene regulation and transcription, replication, mitosis and apoptosis.
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