产品名称
PKA蛋白,重组,10 µg, Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.
biological source
human
recombinant
expressed in E. coli
expressed in BL21 DE3 cells
product line
Upstate®
form
liquid
specific activity
(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
8580 U/mg
shelf life
1 yr
mol wt
Mw 40.7 kDa
purified by
chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
solubility
water: soluble
accession no.
(FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. )
NM_002730.3
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Quality Level
Gene Information
human ... PRKACA(46909581)
Analysis Note
通过体外Kemptide底物的磷酸化进行常规评估
Biochem/physiol Actions
蛋白质靶标:PKA;亚家族靶标:AGC。蛋白激酶是参与真核细胞信号传导网络的重要蛋白。在Hedgehog响应细胞中,PKA通过影响Hedgehog通路信号传导来调节增殖和命运决定,发挥重要作用。PKA有无数与粘附或迁移有关的靶标。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
研究领域:细胞信号传导
产品来源:大肠杆菌BL21 (DE3) pLysS细胞表达的重组人全长PKA,催化亚基α型,未标记蛋白激酶A(PKA)是一种丝氨酸蛋白激酶和异四聚体全酶,靶向丝氨酸和苏氨酸残基。
产品来源:大肠杆菌BL21 (DE3) pLysS细胞表达的重组人全长PKA,催化亚基α型,未标记蛋白激酶A(PKA)是一种丝氨酸蛋白激酶和异四聚体全酶,靶向丝氨酸和苏氨酸残基。
PKA
重组人全长PKA,催化亚基型α,未标记
Other Notes
替代:14-114
比活相关数据,请参阅检验报告以获取该酶的单独批次。
Packaging
也提供250 µg的规格-订购250 µg的规格时,请致电咨询价格和供货情况,参考目录号14-440M。
另提供10 μg装和散装形式
Physical form
磷酸纤维素P11色谱法
Preparation Note
在-70°C下保存1年
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 1
PKA: a portrait of protein kinase dynamics
Taylor SS, et al.
Biochimica et Biophysica Acta, 1697(1-2), 259-269 (2004)
Protein Kinase A Activity and Hedgehog Signaling Pathway
Kotani T
Vitamins and Hormones, 88, 273-291 (2012)
Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Test, 200, 581-596 (1991)
Protein Kinase A in cellular migration?Niche signaling of a ubiquitous kinase
Svec KV and Howe AK
Frontiers in molecular biosciences, 9 (2022)
Protein kinase A
Morrison H
Journal of Separation Science, 173-178 (2021)
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