Ral Assay Reagent (Ral BP1, agarose)

GST fusion-protein, corresponding to residues 397-519 of rat Ral binding protein (RalBP1) bound to agarose. Also referred to as RLIP and RLP; specifically binds to and precipitates RalA-GTP and RalB-GTP from cell lysates.

Product Source: Expressed in E. coli.

Routinely evaluated by Affinity Precipitation/Immunoblot Analysis

300 μg of protein in 600 μL of a 50% agarose slurry in 50 mM Tris, pH 7.5, 200 mM NaCl, 1% Triton X-100, 20 μM PMSF, 1 mM aprotinin prior to the addition of glycerol to 50%. Liquid suspension.

1 year at -20°C

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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