biological source
bovine
form
liquid
mol wt
Mw ~47 kDa
manufacturer/tradename
Upstate®
concentration
1 mg/mL
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Analysis Note
Routinely evaluated by kinase assay
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
General description
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Other Notes
Purity information is lot specific, please consult the certificate of analysis
Physical form
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
ProBond Resin chromatography
Preparation Note
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Vanessa D Stewart et al.
FEBS letters, 596(22), 2952-2973 (2022-09-15)
Myelin-associated glycoprotein (MAG) and Nogo inhibit neurite outgrowth by binding to receptors such as NgR1, PirB and LRP1, and they have also been shown to induce phosphorylation of Smad2, a key intermediate in the transforming growth factor β (TGFβ) signalling
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