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等级
RIA grade
检测方案
≥98% (agarose gel electrophoresis)
形式
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
杂质
≤3.0 ash
≤5.0% moisture
颜色
off-white
溶解性
0.15 M sodium chloride: 10 mg/mL
痕量阳离子
heavy metals: ≤10 ppm
运输
ambient
储存温度
2-8°C
一般描述
牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白，含有17个二硫键。
牛血清白蛋白，组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。在150 mM NaCl中10%的pH值为6.8-7.4。
应用
牛血清白蛋白，组分V(RIA和ELISA级)已用于：
- 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
- 作为Krebs缓冲液组分，用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
- 作为溶剂对照孵育小鼠全血，用于研究体内血小板聚集
生化/生理作用
牛血清白蛋白(BSA)是细胞培养基的重要成分，可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。
警告
毒性：标准处理（A）
重悬
在复溶后，分装并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
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