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分子量
Mw 2673 Da
质量水平
制造商/商品名称
Upstate®
技术
activity assay: suitable (kinase)
NCBI登记号
UniProt登记号
运输
dry ice
应用
GSM (GSK3 substrate peptide) has been used:
- in in vitro glycogen synthase kinase 3 (GSK-3)-3β activity assay to measure the kinase activity of GSK-3
- as a supplement in perfusates in kinase activity assay to determine the effect of the phosphatase-activating domain (PAD) peptide on squid axoplasm
- as a control in Kinase-Glo luminescent kinase assay to measure the influence of ibuprofen treatment on the catalytic activity of the kinase GSK3β
生化/生理作用
Glycogen synthase kinase 3 (GSK3) is a protein kinase, which regulates glycogen metabolism. It regulates various cell functions, like signaling by insulin, growth factors and nutrients, and the specification of cell fates during embryonic development. GSK3 is involved modulating in apoptosis, cell division, and microtubule function.
质量
Routinely evaluated as a substrate for GSK3β (Catalog # 14-306).
外形
1 mg of GSM lyophilized from 1 mL sterile, distilled water. Lyophilized powder.
储存及稳定性
Lyophilized: 2 years at -20°C. Rehydrated: 6 months at -20°C
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
An assay for glycogen synthase kinase 3 (GSK-3) for use in crude cell extracts
Ryves, W. J., et al
Analytical biochemistry, 264, 124-127 (1998)
Nydia Tejeda-Muñoz et al.
Molecular and cellular biology, 36(5), 731-741 (2015-12-30)
The molecular events that drive Wnt-induced regulation of glycogen synthase kinase 3β (GSK-3β) activity are poorly defined. In this study, we found that protein kinase Cζ (PKCζ) and GSK-3β interact mainly in colon cancer cells. Wnt stimulation induced a rapid
Vânia Gonçalves et al.
Oncotarget, 11(47), 4421-4437 (2020-12-15)
A major risk factor promoting tumor development is chronic inflammation and the use of nonsteroidal anti-inflammatory drugs (NSAID), including ibuprofen, can decrease the risk of developing various types of cancer, including colorectal cancer (CRC). Although the molecular mechanism behind the
P Cohen et al.
Nature reviews. Molecular cell biology, 2(10), 769-776 (2001-10-05)
Glycogen synthase kinase 3 (GSK3) was initially described as a key enzyme involved in glycogen metabolism, but is now known to regulate a diverse array of cell functions. The study of the substrate specificity and regulation of GSK3 activity has
W Jonathan Ryves et al.
Biochemical and biophysical research communications, 290(3), 967-972 (2002-01-19)
Lithium inhibits (Li(+)) glycogen synthase kinase-3 (GSK-3) by competition for magnesium (Mg(2+)), but not ATP or substrate. Here, we show that the group II metal ion beryllium (Be(2+)) is a potent inhibitor of GSK-3 and competes for both Mg(2+) and
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