推荐产品
产品名称
Chromolith® RP-18封端, L × I.D. 100 mm x 25 mm Prep column
物料
PEEK column
质量水平
Agency
suitable for USP L1
产品线
Chromolith®
特点
endcapped
包装
1 ea of
标记范围
18% Carbon loading
参数
100 bar pressure
45 °C max. temp.
技术
HPLC: suitable
LC/MS: suitable (Prep LC)
UHPLC-MS: suitable
UHPLC: suitable
色谱柱长度 × 内径
10 cm × 2 mm
基质
monolithic silica
基质活性基团
C18 bonding phase
孔径
12 nm mesoporosity
3 μm macroporosity
工作pH范围
2-7.5
分离技术
reversed phase
储存温度
no temp limit
一般描述
Preparative HPLC involves much higher sample loading volumes than analytical chromatography. Consequently, greater sample throughput and separation speed are essential for optimal productivity. These criteria are best fulfilled by Chromolith® Prep RP-18 endcapped columns. The combination of macro and mesopores maximizes separation efficiency and flow rate, while minimizing resistance.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
应用
- Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith® RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et al., 2020).
- Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith® RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et al., 2017).
- Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith® RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).
分析说明
Pressure: ≤ 20 bar
Theoretical Plates (N/m) (Anthracene): ≥ 40000
Capacity factor (Anthracene): 3.0 - 4.5
Tailing (USP): 0.8 - 1.4 %
separation factor (anthracene/progesterone): 1.9 - 2.5
Theoretical Plates (N/m) (Anthracene): ≥ 40000
Capacity factor (Anthracene): 3.0 - 4.5
Tailing (USP): 0.8 - 1.4 %
separation factor (anthracene/progesterone): 1.9 - 2.5
法律信息
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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