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Merck
CN
所有图片(1)

文件

12-457

Sigma-Aldrich

MYPT1 (654-880)

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UNSPSC代码:
12352200
eCl@ss:
32160405
NACRES:
NA.41

检测方案

≥70% (Coomassie staining, SDS-PAGE)

质量水平

形式

liquid

制造商/商品名称

Upstate®

技术

activity assay: suitable (kinase)

NCBI登记号

UniProt登记号

运输

dry ice

基因信息

human ... PPP1R12A(4659)

一般描述

Product Source: expressed in E. coli
ROK substrate protein corresponding to amino acids 654-880 of human MYPT1 with an N-terminal GST tag. MW = 60 kDa.

生化/生理作用

Protein Target: myosin phosphatase

质量

Routinely evaluated as a substrate in a Protein Kinase Assay.

外形

100mM Tris-HCl pH 8.0, 200mM NaCl, 10mM glutathione and 5μg/ml leupeptin. Frozen solution.

储存及稳定性

1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the vial prior to removing the cap.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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J Feng et al.
The Journal of biological chemistry, 274(52), 37385-37390 (1999-12-22)
It is clear from several studies that myosin phosphatase (MP) can be inhibited via a pathway that involves RhoA. However, the mechanism of inhibition is not established. These studies were carried out to test the hypothesis that Rho-kinase (Rho-associated kinase)
G Totsukawa et al.
The Journal of cell biology, 144(4), 735-744 (1999-02-26)
It has been demonstrated previously that during mitosis the sites of myosin phosphorylation are switched between the inhibitory sites, Ser 1/2, and the activation sites, Ser 19/Thr 18 (Yamakita, Y., S. Yamashiro, and F. Matsumura. 1994. J. Cell Biol. 124:129-
Yi-Ju Lee et al.
Journal of cellular physiology, 220(2), 476-484 (2009-04-25)
Cellular responses are determined by a number of signaling cues in the local microenvironment, such as growth factors and extracellular matrix (ECM). In cultures of mammary epithelial cells (MECs), functional differentiation requires at least two types of signal, lactogenic hormones

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