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1.24568

蛋白酶 K

(from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14

别名:

蛋白酶 K 来源于林伯氏白色念球菌, 旋毛虫属碱性蛋白酶, 肽链内切酶

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关于此项目

化学文摘社编号:
39450-01-6
UNSPSC Code:
12352204
EC Index Number:
254-457-8
NACRES:
NA.51
EC 号:
3.4.21.64 (BRENDA, IUBMB)
MDL number:
MFCD00132129

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产品名称

蛋白酶 K, (from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14

form

solid

mol wt

28.93 kDa

pH

6.2-6.8 (20 °C, 10 g/L in H2O)

density

1.1 g/cm3

bulk density

35 kg/m3

storage temp.

2-8°C

Quality Level

400

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Analysis Note

外观（颜色）：白色
外观（描述）：冻干粉末
活性（血红蛋白； pH 7.5；37 °C）： ≥ 30.0 mAnsonU/mg
比活力（以蛋白质计算）： ≥ 40 mAnsonU/mg
脱氧核糖核酸酶（切口活性；pBR 322；6 H；37 °C）：未检出
RNA酶（RNA；2 h；37°C）：未检出

Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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