产品名称
Fractogel® EMD DEAE (M), weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
ligand
diethylaminoethyl
Quality Level
product line
Fractogel®
form
resin
parameter
170 cm/hr flow rate
8 bar max. pressure
matrix active group
methacrylate
mean particle size
40-90 μm
capacity
100 mg binding capacity (BSA/mg of resin)
transition temp
flash point 35 °C (calculated)
density
1.430 g/cm3
bulk density
1000 kg/m3
application(s)
gene therapy
mAb
vaccine development
separation technique
weak anion exchange
storage temp.
2-30°C
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General description
Packaging
- 1.16883.0100: Fractogel® EMD DEAE (M) 树脂 100ml
- 1.16883.0010: Fractogel® EMD DEAE (M) 树脂 10ml
- 1.16883.0500: Fractogel® EMD DEAE (M) 树脂 500ml
- 1.16883.5000: Fractogel® EMD DEAE (M) 树脂 5L
Legal Information
Analysis Note
显微镜评估: 均匀的球状颗粒，无结块，无细屑
可萃取物（水）: ≤ 0.03 %
铈: ≤ 1 µg/g
压降(柱：内径=1.6 cm, 长度=10 cm, 5ml/min): ≤ 1 bar
粒径（d10）：37 - 45 µm
粒径（d50）：48 - 60 µm
粒径（d90）：63 - 77 µm
菌落形成单位(TAMC + TYMC): ≤ 100 CFU/ml
内毒素： ≤ 1.00 EU/ml
蛋白质结合能力（牛血清白蛋白）: 80 - 120 mg/ml
功能测试（b:a）: ≤ 0.25
功能测试: 伴白蛋白和人血清白蛋白的更分离
Features and Benefits
- 与大病毒和质粒DNA的稳定结合
- 高选择性和纯度下的均匀结合（homogenous binding）
- 较低的洗脱体积带来最高的纯度水平
- 兼容 含150 mM NaCl储存溶液的2.5 % (v/v) 水性苄醇溶液
由于其滴定行为，离子交换容量在pH 2 至pH 9.5的范围内有效。蛋白质分离基于蛋白表面的负电荷区与载体之间的可逆静电相互作用。当缓冲液的 pH 值比蛋白质的等电点高1个单位左右时，蛋白质会在Fractogel® EMD DEAE上被高效地截留。
结合强度取决于以下因素：
- 缓冲体系
- 决定蛋白质表面电荷的缓冲液的pH值
- 交换剂官能团的电离程度
- 反离子的浓度
- 载体的电荷密度（蛋白结合能力）
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
95.0 °F
flash_point_c
35 °C
法规信息
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