一般描述
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Antibody Target Gene Symbol: ACTA2 Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN Entrez Gene Name: actin, alpha 2, smooth muscle, aorta Hu Entrez ID: 59 (Related Antibodies: 113200) Rat Entrez ID: 81633
This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
免疫原
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
应用
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
分析说明
Negative Control
Fibroblasts, striated muscle, or myocardium
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
其他说明
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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