推荐产品
方案
≥98% (HPLC)
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
protect from light
颜色
burnt orange
溶解性
DMF: 1 mg/mL
H2O: 1 mg/mL
ethanol: 1 mg/mL
运输
ambient
储存温度
15-25°C
InChI
1S/C17H19N3.ClH/c1-19(2)13-8-9-15-12(10-13)11-14-16(18-15)6-5-7-17(14)20(3)4;/h5-11H,1-4H3;1H
InChI key
ASKSWIKIIFUEOI-UHFFFAOYSA-N
一般描述
A cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
A cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
生化/生理作用
Cell permeable: yes
Primary Target
Interacts with DNA and RNA
Interacts with DNA and RNA
Product does not compete with ATP.
警告
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
重悬
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
其他说明
Gonzalez, K., et al. 1995. Curr. Eye Res.14, 269.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
Muro-Cacho, C.A., et al. 1995. J. Immunol.154, 5555.
Olivier, R. 1995. Methods Enzymol.251, 270.
Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA91, 9165.
Darzynkiewicz, Z. 1994. Methods Cell. Biol.41, 401.
Delic, J., et al. 1991. Exp. Cell Res.194, 147.
El-Naggar, A.K., et al. 1991. Cytometry12, 330.
Lopez, F., et al. 1991. Cytometry12, 42.
Gurrieri, S., et al. 1990. Biochemistry29, 3396.
Hermansen, D.K., et al. 1989. Cytometry10, 739.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Muta. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Simultaneous analysis of cellular RNA and DNA content.
Z Darzynkiewicz
Methods in cell biology, 41, 401-420 (1994-01-01)
J Delic et al.
Experimental cell research, 194(1), 147-153 (1991-05-01)
The ability of a "vital" dye, acridine orange (AO), to intercalate into the DNA of living cells was investigated by quantitative intensified fluorescence microscopy and digital imaging under various conditions of dye concentration, excitation light intensity, and ionic concentration. Our
C A Muro-Cacho et al.
Journal of immunology (Baltimore, Md. : 1950), 154(10), 5555-5566 (1995-05-15)
The occurrence of in vivo apoptosis was investigated in lymph node sections obtained from HIV-infected persons at different stages of disease. The degree of apoptosis in lymph nodes from HIV-infected individuals was compared with that observed in lymph nodes obtained
A K el-Naggar et al.
Cytometry, 12(4), 330-335 (1991-01-01)
We investigated the ability of single- and double-stranded RNA measurements to discriminate between neoplastic and non-neoplastic solid tissues. Sixty-one solid nonhematopoietic neoplasms, 10 reactive non-neoplastic lesions, and 26 normal tissue samples were the test materials. Single-stranded ribonucleic acid (s-RNA) levels
S Gurrieri et al.
Biochemistry, 29(13), 3396-3401 (1990-04-03)
The dynamics of individual DNA molecules undergoing orthogonal field alternating gel electrophoresis (OFAGE) have been studied by use of T2 DNA molecules labeled with a dye and visualized with a fluorescence microscope. The mechanism of reorientation used by a molecule
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