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质量水平
形式
liquid
组成
Water, 74.5-75.5% Karl Fischer
浓度
24.5-25.5% (trifluoroacetic acid)
密度
1.12 g/cm3 at 20 °C
储存温度
15-25°C
SMILES字符串
OC(=O)C(F)(F)F
InChI
1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
InChI key
DTQVDTLACAAQTR-UHFFFAOYSA-N
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应用
Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).
分析说明
Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
警示用语：
Danger
危险声明
危险分类
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
储存分类代码
8B - Non-combustible, corrosive hazardous materials
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
危险化学品
Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13
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