1.08218
三氟乙酸 溶液
(25% solution in water) for protein sequenation
别名:
TFA
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关于此项目
线性分子式:
CF3COOH
化学文摘社编号:
分子量:
114.02
NACRES:
NB.21
UNSPSC Code:
12352106
Beilstein/REAXYS Number:
5446934
MDL number:
Concentration:
24.5-25.5% (trifluoroacetic acid)
Form:
liquid
产品名称
三氟乙酸 溶液, (25% solution in water) for protein sequenation
SMILES string
OC(=O)C(F)(F)F
InChI key
DTQVDTLACAAQTR-UHFFFAOYSA-N
InChI
1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
form
liquid
composition
Water, 74.5-75.5% Karl Fischer
concentration
24.5-25.5% (trifluoroacetic acid)
density
1.12 g/cm3 at 20 °C
storage temp.
15-25°C
Quality Level
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Analysis Note
Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
Application
Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
存储类别
8B - Non-combustible, corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
危险化学品
此项目有
Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13
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