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Merck
CN
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1.07393

Sigma-Aldrich

蛋白酶 K (来源于真菌)，用于生物化学

(from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14

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别名:
ProK, native proteinase K solution, non-specific protease
UNSPSC代码:
12352204
NACRES:
NA.51

质量水平

400

形式

liquid

pH值（酸碱度）

7.5 (25 °C in H2O, undiluted)

密度

1.10 g/cm3 at 20 °C

储存温度

2-8°C

一般描述

蛋白酶K是一种肽链内切酶，属于蛋白酶的枯草杆菌蛋白酶家族。多肽链含有278 个氨基酸和催化三联体Asp39、His69、Ser224。

应用

蛋白酶K已用于：
  • 免疫沉淀样品的去交联
  • 脱氧核糖核苷酸末端转移酶dUTP缺口末端标记（TUNEL）测定中的多聚-L-赖氨酸包被的结肠组织载玻片的处理
  • 原位杂交

生化/生理作用

蛋白酶 K 催化酯和肽键的水解。它和水化的非水溶剂一起用于合成肽。蛋白酶 K用于打破福尔马林固定产生的交联键，并使引物和聚合酶的靶序列暴露出来。

分析说明

外观（颜色）：无色
外观（清晰度）：透明
活性（血红蛋白；pH 7.5；37 °C）： ≥ 600 mAnsonU/ml
比活度（以蛋白质计）： ≥ 40.0 mAnsonU/mg
DNases（切割活性；pBR 322；6 h；37 °C）：无法检测
RNases（RNA；2 h；37°C）：无法检测
菌落数（需氧菌）： ≤ 10 CFU/ml

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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F Chavagnat et al.
Applied and environmental microbiology, 65(7), 3001-3007 (1999-07-02)
The general aminopeptidase PepN from Streptococcus thermophilus A was purified to protein homogeneity by hydroxyapatite, anion-exchange, and gel filtration chromatographies. The PepN enzyme was estimated to be a monomer of 95 kDa, with maximal activity on N-Lys-7-amino-4-methylcoumarin at pH 7
María Isabel Navarro-Mendoza et al.
Current biology : CB, 29(22), 3791-3802 (2019-11-05)
Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A

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