HPTLC plates, Silica gel 60 DIOL F₂₅₄s

25 Glass plates 20 x 10 cm

Our NH2, CN and DIOL -modified silica sorbents are less polar then conventional silica phases, making them ideal for separating hydrophilic or charged substances. Our moderately polar cyano- and diol-modified silica plates can be used for both normal phase and reversed phase systems. An alternative to PEI cellulose, amino-modified NH2 plates provide weak basic ion exchange characteristics with special selectivity for charged compounds.

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g


Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g


d 50 (laser diffraction, size distribution): 5 - 7 µm


Layer thickness: 150 - 200 µm


Deviation of layer thickness per plate: ≤ 35 µm


Chromatographic testings:


A) Steroidtest, straight phase


hRf-values


- hydrocortisone, straight phase:


4 - 8


- Reichstein S, straight phase: 11 - 18


- Methyltestosterone, straight phase: 27 - 36


B) Steroid test, reversed phase


- methyltestosterone, steroid test: 40 - 65


- Reichstein S, steroid test: 50 - 70


- hydrocortisone, steroid test: 60 - 80


Typical value determined on an activated plate (120°C, 15 min)


Eluent a) petroleum benzine 40-60°/acetone (80/20 v/v)


Eluent b) acetone/water (30/70 v/v)