Oil red O (C.I. 26125)

The dry dye Oil red O (C.I. 26125) for microscopy Certistain^®, is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues, in joint pathology or in cytological sample material. This Oil red O solution is ready-to-use and results in a brilliant, bright red staining of lipids, while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Cat. no. 105175).
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids.
As Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications.

For microscopy Certistain^®

<tr><td width=\25%\>Dye content (spectrophotometrically)</td><td width=\75%\>≥ 80 %</td></tr><tr><td width=\25%\>Identity (UV/VIS-Spectrum)</td><td width=\75%\>passes test</td></tr><tr><td width=\25%\>Absorption maximum λ max (chloroform)</td><td width=\75%\>528 - 534 nm</td></tr><tr><td width=\25%\>Spec. Absorptivity A 1%/1cm (λ<SUB>m</SUB><SUB>a</SUB><SUB>x</SUB>; 0.01 g/l; chloroform)</td><td width=\75%\>725 - 910</td></tr><tr><td width=\25%\>TLC-Test</td><td width=\75%\>passes test</td></tr><tr><td width=\25%\>Loss on drying (110 °C)</td><td width=\75%\>≤ 8 %</td></tr><tr><td width=\25%\>Suitability for microscopy</td><td width=\75%\>passes test</td></tr>

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