Contact TVC

Count the number of colonies on both sides of the paddle- results should not differ by a factor greater than 2, where the colonies on side A will appear mainly in red. In the presence of TTC a few microorganisms may grow less profusely. Always go with the higher counts in case they are not identical on both sides. This represents the microbial load for the test.

Sterility: passes test
pH-value/side one (25 °C): 6.7 - 7.3
pH-value/side two (25 °C): 6.7 - 7.3
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): fair/good
- Candida albicans ATCC 10231 (WDCM 00054): none/poor
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Candida albicans ATCC 10231 (WDCM 00054): good
- Bacillus subtilis ATCC 6633 (WDCM 00003): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Incubation: 48 hrs.; 35 °C; aerobic; Aspergillus up to 72 hrs.

Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.

(Total Viable Counts) Envirocheck^™

Envirocheck contact total viable count, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Nutrient Agar to determine the total viable count of bacteria in sample. Slide A is Coated with Nutrient Agar containing 0.05% Triphenyl Tetrazolium Chloride (TTC) and Slide B Coated with Nutrient Agar. The growth of bacteria on slide A is indicated by red colonies due to the formation of red formazan dye when TTC is reduced.

ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany