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Merck
CN

07-896-I

Anti-phospho-Rac1/cdc42 (Ser71) Antibody

from rabbit, purified by affinity chromatography

别名:

Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, phospho-Rac1/cdc42 (Ser71)

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

Anti-phospho-Rac1/cdc42 (Ser71) Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), orangutan (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_008839

UniProt accession no.

P63000

shipped in

wet ice

target post-translational modification

phosphorylation (pSer71 )

Quality Level

100

Gene Information

human ... RAC1(5879)

Analysis Note

Evaluated by Western Blotting in EGF treated A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rac1/cdc42 (Ser71) in 10 µg of EGF treated A431 cell lysate.

Application

Detect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.
Immunoprecipitation Analysis: 2.5µg from a representative lot immunoprecipitated phospho-Rac1/cdc42 (Ser71) in EGF treated A431 cell lysate.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.
~25 kDa observed

Immunogen

Epitope: Near N-Terminus
KLH-conjugated linear peptide corresponding to human phospho-Rac1/cdc42 (Ser71) near the N-Terminus.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

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