07-875
Anti-phospho-PKCδ (Ser664) Antibody
Upstate®, from rabbit
别名:
PRKCD, PKC delta, PKCd, Protein Kinase C, delta
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
western blot
Technique(s):
western blot: suitable
Citations:
2
Uniprot accession no.:
产品名称
Anti-phospho-PKCδ (Ser664) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer664)
Quality Level
Gene Information
human ... PRKCD(5580)
Analysis Note
Routinely evaluated by immunoblot.
Application
Detect phospho-PKCδ (Ser664) using this Anti-phospho-PKCδ (Ser664) Antibody validated for use in WB.
Immunoblot Analysis: A 1:1,000 dilution of this lot detected phospho-PKCδ (Ser664) in RIPA lysate of PMA-treated K562 cells.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Predicted to cross-react more weakly with mouse based on sequence homology, but have not been tested.
Recognizes phospho-PKCδ (Ser664), weaker with with mouse and rat PKCδ (Ser662) and PKCα (Ser657) and PKCβII (Ser662).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
78 kDa
Immunogen
Peptide corresponding to amino acid region encompassing the human phospho-PKCδ (Ser664).
Physical form
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified
Preparation Note
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Patrick Kwok-Shing Ng et al.
Cancer cell, 33(3), 450-462 (2018-03-14)
The functional impact of the vast majority of cancer somatic mutations remains unknown, representing a critical knowledge gap for implementing precision oncology. Here, we report the development of a moderate-throughput functional genomic platform consisting of efficient mutant generation, sensitive viability
Steven M Hill et al.
Cell systems, 4(1), 73-83 (2016-12-27)
Signaling networks downstream of receptor tyrosine kinases are among the most extensively studied biological networks, but new approaches are needed to elucidate causal relationships between network components and understand how such relationships are influenced by biological context and disease. Here, we investigate
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