生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
制造商/商品名称
Upstate®
技术
ChIP: suitable (ChIP-seq)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
acetylation (Lys 4)
基因信息
human ... H3F3B(3021)
特异性
组蛋白H3在Lys4上乙酰化时
预计具有广泛的物种交叉反应性
免疫原
KLH偶联合成肽包含序列 ...RT[AcK]Q...，其中[AcK]对应于人组蛋白H3的乙酰赖氨酸4
应用
建议的稀释度：
蛋白质印迹：1:1000 - 1:10,000
蛋白质印迹：1:1000 - 1:10,000
抗乙酰基组蛋白H3（Lys 4）抗体是一种兔多克隆抗体，用于检测乙酰基组蛋白H3（Lys 4），也称为H3K4Ac，组蛋白H3（乙酰基K4）& 已在Mplex、WB、PIA、ChIP、ChIP-seq、DB中得到验证。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
质量
已通过免疫印迹进行常规评估。
目标描述
Mr ~17kDa
外形
100 μL免疫亲和纯化的兔IgG，溶于在含有0.1 M Tris-甘氨酸（pH 7.4）和0.15 M NaCl，并含有0.05%叠氮化钠和30%甘油的缓冲液中
免疫亲和纯化
储存及稳定性
自运送之日起在-20°C下保存1年
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 1
Federico Forneris et al.
The Journal of biological chemistry, 281(46), 35289-35295 (2006-09-22)
Human lysine-specific demethylase (LSD1) is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys4 of histone H3 (H3-K4). We used a combination of in vivo and in vitro experiments to characterize the substrate specificity and recognition
Runsheng He et al.
Nucleic acids research, 45(11), 6427-6441 (2017-04-13)
Epigenetic regulation of chromatin plays a critical role in controlling embryonic stem (ES) cell self-renewal and pluripotency. However, the roles of histone demethylases and activating histone modifications such as trimethylated histone 3 lysine 4 (H3K4me3) in transcriptional events such as
Cheng-Yu Yang et al.
Oncotarget, 8(20), 33756-33769 (2017-04-20)
Aberrant activation of histone lysine-specific demethylase (LSD1) increases tumorigenicity; hence, LSD1 is considered a therapeutic target for various human cancers. Although melatonin, an endogenously produced molecule, may defend against various cancers, the precise mechanism involved in its anti-oral cancer effect
Ya-Wei Chen et al.
Cancer, 119(24), 4259-4267 (2013-12-05)
Patterns of global histone modifications have been suggested to be predictors of clinical outcome in many cancers. However, the role of global histone modification patterns in oral squamous cell carcinoma (OSCC) is unclear. A retrospective clinicopathologic analysis was undertaken of
Oral administration of the pimelic diphenylamide HDAC inhibitor HDACi 4b is unsuitable for chronic inhibition of HDAC activity in the CNS in vivo.
Beconi, M; Aziz, O; Matthews, K; Moumne, L; O'Connell, C; Yates, D; Clifton, S; Pett et al.
Testing null
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