07-502
Anti-p67-phox (mouse) Antibody
Upstate®, from rabbit
别名:
67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, chronic granulomatous disease, autosomal 2, neutrophil cytosol factor 2, neutrophil cytosolic factor 2, neutrophil cytosolic factor 2 (65kD, chronic granuloma
产品名称
Anti-p67-phox (mouse) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ncf2(17970)
rat ... Ncf2(364018)
Analysis Note
Control
Rat brain tissue, RAW 264.7 cell lysate.
Rat brain tissue, RAW 264.7 cell lysate.
Evaluated by western blot in RIPA lysates from RAW 264.7 cells.
Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.
Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.
Application
Detect p67-phox (mouse) using this Anti-p67-phox (mouse) Antibody validated for use in WB, IH.
Immunohistochemistry: This antibody has been demonstrated by an independent laboratory to detect p67-phox in male rat brain cortex tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Biochem/physiol Actions
Recognizes p67-phox, MW ~67 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
67 kDa
Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.
Immunogen
KLH-conjugated, synthetic peptides corresponding to the first 15 amino acids from the N-terminal and the last 15 amino acids on the C-terminal of mouse p67-phox.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
K Mizuki et al.
European journal of biochemistry, 251(3), 573-582 (1998-03-07)
The phagocyte NADPH oxidase is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The formation of the active oxidase complex at the membrane requires translocation of the Rac GTPase and two specialized cytosolic proteins that harbor SH3
Ying-Ying Chen et al.
International journal of molecular sciences, 23(13) (2022-07-10)
Chronic hyperglycemia triggers an abnormal rise in reactive oxygen species (ROS) that leads to blindness in patients with diabetes mellitus (DM) and cataracts. In this study, the effects of dapagliflozin, metformin and resveratrol on ROS production were investigated in lens
The superoxide-generating NADPH oxidase: structural aspects and activation mechanism.
Vignais, P V
Cellular and Molecular Life Sciences, 59, 1428-1459 (2002)
Ming Gao et al.
International journal of molecular medicine, 46(3), 1239-1248 (2020-06-26)
Diabetes‑associated cognitive decline is a recently identified a potential complication of diabetes. The present study was designed to examine the effects of troxerutin, a potent antioxidant, on cognitive function in rats with streptozotocin‑induced diabetes and to further explore the potential
PtdIns3P binding to the PX domain of p40phox is a physiological signal in NADPH oxidase activation.
Ellson, C; Davidson, K; Anderson, K; Stephens, LR; Hawkins, PT
The Embo Journal null
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