Anti-p67-phox (mouse) Antibody

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

Recognizes p67-phox, MW ~67 kDa.

KLH-conjugated, synthetic peptides corresponding to the first 15 amino acids from the N-terminal and the last 15 amino acids on the C-terminal of mouse p67-phox.

Detect p67-phox (mouse) using this Anti-p67-phox (mouse) Antibody validated for use in WB, IH.

Immunohistochemistry: This antibody has been demonstrated by an independent laboratory to detect p67-phox in male rat brain cortex tissue.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis: 0.5-2.0 µg/mL of this antibody detected p67-phox in RIPA lysates from RAW 264.7 cells.

67 kDa

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain tissue, RAW 264.7 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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