产品名称
抗髓过氧化物酶抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
flow cytometry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MPO(4353)
Analysis Note
通过蛋白质印迹对Jurkat细胞裂解液进行了评估。
蛋白质印迹分析：1 µg/mL的该抗体在10 µg Jurkat细胞裂解液中检测到髓过氧化物酶重链。
蛋白质印迹分析：1 µg/mL的该抗体在10 µg Jurkat细胞裂解液中检测到髓过氧化物酶重链。
Application
抗髓过氧化物酶抗体（目录号07-496）是一种高度特异性的兔多克隆抗体，靶向髓过氧化物酶，并通过流式细胞术和蛋白质印迹法进行了测试。
流式细胞分析：0.1 µg代表性批次在4%多聚甲醛固定的0.2%Triton X-100透化的HL-60细胞中检测到髓过氧化物酶免疫反应性。
Biochem/physiol Actions
免疫原序列存在于髓过氧化物酶的前体和成熟形式中（无a.a.1-48，a.a.1-154，或a.a.1-164），以及裂解的重链中（a.a.279-745），但不存在轻链中（a.a.165-278）。
General description
观察到约92/60 kDa（糖基化前体/重链）。83.87/73.85/87.25 kDa（亚型H17/H14/H7前体），78.94/82.32 kDa（亚型H17/H7，去除a.a.1-48前肽），66.45/66.45/69.83 kDa（亚型H17/H14/H7，去除a.a.1-154），65.48/65.48/68.86 kDa（亚型H17/H7亚型H17/H14/H7，去除a.a.1-164），52.51 kDa（重链；a.a.279-745）。在一些裂解液中可能会观察到未表征的条带。
髓过氧化物酶（EC 1.11.2.2；UniProt P05164）由人的MPO基因（基因ID 4353）编码。髓过氧化物酶/MPO是在髓样分化过程中合成的血红素蛋白，构成嗜中性粒细胞嗜天青颗粒的主要成分。髓过氧化物酶以具有信号肽序列的745 a.a.单链前体（a.a.1-48）生成，随后被切割成轻链（a.a.165-278）和重链（a.a.279-745）。成熟的髓过氧化物酶是由2条轻链和2条重链组成的四聚体。MPO催化过氧化氢依赖性氧化和次氯酸的形成，而次氯酸对嗜中性粒细胞的杀微生物活性至关重要。
Immunogen
对应于人髓过氧化物酶重链区域内序列的KLH偶联线性肽。
Other Notes
替代：07-496
浓度：请参考特定批次的数据表。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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