Anti-PPARγ Antibody

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARα is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. PPARβ is the most widely distributed subtype and is often expressed at high levels. PPARγ is predominantly seen in adipose tissue where it plays a critical role in regulating adipocyte differentiation.

Predicted to cross-react with rat, pig, rabbit, rhesus monkey, mink and other vertebrates based on sequence conservation.

Recognizes PPARγ1 and 2, Mr 55-60 kDa. A non-specific protein was also detected, Mr 47 kDa after longer exposure.

KLH-conjugated, synthetic peptide (GEDKIKFKHITPLQEQ) corresponding to amino acids 256-271 of human PPARγ1 and amino acids 287-302 of human PPARγ2. The immunizing sequence is identical in mouse and rat.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-PPARγ Antibody (Rabbit Polyclonal Antibody) validated in WB to detect PPARγ also known as Nuclear receptor subfamily 1 group C member 1, peroxisome proliferator activated receptor alpha.

Evaluated by western blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes.

Western Blot Analysis:
1-5 μg/mL of this antibody detected PPARγ protein in RIPA lysates from 3T3/L1 cells differentiated into adipocyte

~67 kDa

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

Stable for 1 year at -20ºC from date of receipt.

Control
Human breast carcinoma tissue, rat skeletal muscle extract or U-937 cell lysate.

Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.

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