推荐产品
生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
mouse, chicken, human
制造商/商品名称
Upstate®
技术
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
monomethylation (Lys9)
基因信息
human ... HIST1H3F(8968)
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Broad species cross-reactivity is expected based on sequence homology.
应用
Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a purified rabbit polyclonal that detects Histone H3 monomethylated on lysine 9 (H3K9me1). This Ab is specificity verified by dot blot (DB), and validated in ICC, PIA, WB. The small pack size is ideal for screening applications.
质量
Routinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
目标描述
17 kDa
外形
Format: Purified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
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