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Merck
CN
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主要文件

07-2189

Sigma-Aldrich

Anti-CAPZIP Antibody

from rabbit, purified by affinity chromatography

别名:

RCSD domain containing 1, capZ-interacting protein, Protein kinase substrate CapZIP, protein kinase substrate MK2S4, RCSD domain-containing protein 1

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702

生物来源

rabbit

质量水平

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human

技术

immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

wet ice

基因信息

human ... RCSD1(92241)

一般描述

CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.

免疫原

GST-tagged recombinant protein corresponding to human CAPZIP.

应用

Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.
Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

质量

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.

目标描述

~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa

外形

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Jurkat cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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