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生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
技术
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
基因信息
human ... RCSD1(92241)
一般描述
CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.
免疫原
GST-tagged recombinant protein corresponding to human CAPZIP.
应用
Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.
Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
质量
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.
目标描述
~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Jurkat cell lysate
Jurkat cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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