Anti-phospho-FOXO1 Antibody (Thr24)

Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24.

This antibody recognizes FOXO1 phosphorylated at Thr24.

Epitope: Phosphorylated Thr24

KLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24.

Research Category
Signaling

Research Sub Category
Transcription Factors

Use Anti-phospho-FOXO1 (Thr24) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-FOXO1 (Thr24) also known as Forkhead box protein O1.

Evaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.

Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated.

~78 kDa observed.
Calculated molecular weight is 70 kDa FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate untreated and BMP2-treated

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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