Anti-phospho PIX Antibody (Ser340)

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

This antibody recognizes PIX phosphorylated at Ser340.

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

~ 75 kDa

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

Control
PIX transfected cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.