Anti-MBD1 Antibody, CT

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, regulates gene expression and is critical for proper development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that each contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity.

Not tested in other species.

This antibody recognizes human MBD1.

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human MBD1.

Anti-MBD1 Antibody, C-terminus is a Rabbit Polyclonal Antibody for detection of MBD1 also known as CXXC-type zinc finger protein 3 & has been validated in WB, ELISA.

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis:
This antibody recognizes MBD1 on 10 µg of HeLa cell lysate.

~61 kDa

Protein G Chromatography

Format: Purified

Purified rabbit polyclonal in buffer containing 0.09% NaN3.

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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